Down-regulation of complement genes in lipopolysaccharide-challenged zebrafish (Danio rerio) larvae exposed to Indonesian propolis

Abstract Although propolis has been reported for having anti-inflammatory activities, its effects on complement system not much studied. This research was conducted to find out the of Indonesian expression levels C3, C1r/s, Bf, MBL, and C6 in zebrafish larvae which were induced by lipopolysaccharide (LPS). Counting macrophages migrating yolk sac liver histology carried out. Larvae divided into four groups: CON (cultured E3 medium only), LPS a containing 0.5 μg/L LPS), LPSIBU LPS, then treated with 100 ibuprofen 24 hours), LPSPRO immersed 14,000 hours) groups. The results showed that gene from groups generally lower than group. number macrophage migrations group also Histological structure all considered normal. study shows potential be used as an alternative substitution NSAIDs.